Isotopic Labeling of Proteins Using Cell-Free Synthesis for NMR
Cell-free protein synthesis achieves isotopic labeling precision that living cells cannot.
Priya Venkataraman
Section
Production Workflows
7 stories in Production Workflows.
Bispecific and Multispecific Antibody Fragment Production in CFPS
Cell-free synthesis overcomes structural barriers that kill bispecific antibody fragment production.
Marcus Treviño
SPR and Biophysical Assay Protein Preparation Using CFPS
Cell-free protein synthesis enables direct SPR analysis of difficult-to-express proteins.
Priya Venkataraman
CFPS Reaction Scale-Up Without Proportional Cost Increase
Optimizing yield, not volume, cuts CFPS costs per gram.
Danielle Osei-Bonsu
Protein Purification Strategies Downstream of Cell-Free Synthesis
Designing purification steps at the reaction stage prevents failures on the bench.
Soren Althoff
Benchmarking CFPS Yield Against E. Coli Recombinant Expression
Cell-free synthesis beats E. coli for toxic proteins but loses on cost at scale.
Marcus Treviño
Batch CFPS Versus Continuous Exchange Format for Milligram Yields
Continuous exchange format extends reaction time to boost eukaryotic protein yields tenfold.
Marcus Treviño